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Sample GSM1602159 Query DataSets for GSM1602159
Status Public on Feb 04, 2015
Title Homo_N1-2
Sample type RNA
Source name Adjacent normal brain tissue
Organism Homo sapiens
Characteristics individual: patient 1
tissue: Adjacent normal brain tissue
gender: male
age(years): 59
Treatment protocol The tissues were obtained from the operation room and were snap-frozen immediately, stored at -80℃.
Growth protocol A totle of 3 GBM tissues and the matched adjacent normal brain tissues were obtained from patients who underwent primary surgical treatment according to clinical protocols.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250ng total RNA.
Hybridization protocol Following fragmentation, 5.5 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Transcriptome Array, 1 ug of cRNA were hybridized for 16 hr at 48℃ on Affymetrix GeneChip miRNA Array were washed and stained in the affymetrix GeneChip Fluidics Station 450.
Scan protocol GeneChips were scanned using the affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data from Adjacent normal brain tissue.
Data processing The data were analyzed with Affymetrix ExpressionConsole-1_4_0 (EC 5.0) using Robust Multichip Average (RMA) as normalization method.
Submission date Feb 04, 2015
Last update date Feb 04, 2015
Contact name Weitao Man
Phone +8618201060638
Organization name Fuxing Hospital affiliated to Capital Medical University
Street address Fuxing Hospital affiliated to Capital Medical University,Beijing,China
City Beijing
ZIP/Postal code 100036
Country China
Platform ID GPL19117
Series (1)
GSE65626 Expression data from glioblastoma tissue and matched adjacent normal tissue

Data table header descriptions
VALUE log2 RMA signal

Data table
MIMAT0000062_st 12.69419 P
MIMAT0004481_st 0.005647689 A
MIMAT0010195_st 0.3942125 A
MIMAT0000063_st 14.20581 P
MIMAT0004482_st 2.095361 P
MIMAT0000064_st 13.58006 P
MIMAT0026472_st 0.4293709 A
MIMAT0000065_st 12.4488 P
MIMAT0004484_st 0.4797527 A
MIMAT0000066_st 12.20817 P
MIMAT0004485_st 1.56475 P
MIMAT0000067_st 8.749269 P
MIMAT0004486_st 2.298965 P
MIMAT0004487_st 0.4797527 A
MIMAT0000068_st 8.23126 P
MIMAT0004488_st 0.134789 A
MIMAT0000069_st 12.65523 P
MIMAT0004489_st 0.07251482 A
MIMAT0000070_st 10.61015 P
MIMAT0000071_st 5.48827 P

Total number of rows: 6658

Table truncated, full table size 178 Kbytes.

Supplementary file Size Download File type/resource
GSM1602159_Homo_N1-2.CEL.gz 768.7 Kb (ftp)(http) CEL
Processed data included within Sample table

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