|
Status |
Public on Feb 05, 2015 |
Title |
MRT560Gy-irradiated tumour 48hr_07D40 IT |
Sample type |
RNA |
|
|
Source name |
MRT560Gy-irradiated tumour 48hr
|
Organism |
Mus musculus |
Characteristics |
strain background: BALB/c age: 6-8 months cell line: EMT6.5 cell type: mouse breast tumor cell line treatment group: MRT560Gy-irradiated 48hr
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 4
|
Data processing |
Illumina data was formatted into a Partek project data file. Partek Genomics Suite version 6.6 was used for normalization and analysis. Robust multichip average algorithm (RMA) background correction and qunatile normalization were applied to probe intensities.
|
|
|
Submission date |
Feb 04, 2015 |
Last update date |
Feb 05, 2015 |
Contact name |
Yuqing Yang |
E-mail(s) |
yangyq0577@gmail.com
|
Phone |
61 422339209
|
Organization name |
University of Melbourne
|
Street address |
Royal Women's Hospital Level 7, 20 Flemington Rd
|
City |
Parkville |
State/province |
Victoria |
ZIP/Postal code |
3052 |
Country |
Australia |
|
|
Platform ID |
GPL19738 |
Series (1) |
GSE65625 |
Genome-Wide Transcription Responses to Synchrotron Microbeam Radiotherapy |
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