|
| Status |
Public on Feb 05, 2015 |
| Title |
MRT560Gy-irradiated tumour 24hr_07D48 IT |
| Sample type |
RNA |
| |
|
| Source name |
MRT560Gy-irradiated tumour 24hr
|
| Organism |
Mus musculus |
| Characteristics |
strain background: BALB/c
age: 6-8 months
cell line: EMT6.5
cell type: mouse breast tumor cell line
treatment group: MRT560Gy-irradiated 24hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 4
|
| Data processing |
Illumina data was formatted into a Partek project data file. Partek Genomics Suite version 6.6 was used for normalization and analysis. Robust multichip average algorithm (RMA) background correction and qunatile normalization were applied to probe intensities.
|
| |
|
| Submission date |
Feb 04, 2015 |
| Last update date |
Feb 05, 2015 |
| Contact name |
Yuqing Yang |
| E-mail(s) |
yangyq0577@gmail.com
|
| Phone |
61 422339209
|
| Organization name |
University of Melbourne
|
| Street address |
Royal Women's Hospital Level 7, 20 Flemington Rd
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL19738 |
| Series (1) |
| GSE65625 |
Genome-Wide Transcription Responses to Synchrotron Microbeam Radiotherapy |
|
