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Sample GSM1601

Query DataSets for GSM1601

Status

Public on May 01, 2002

Title

6.2.b-BY

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =

Submission date

Apr 26, 2002

Last update date

May 28, 2005

Contact name

Rachel Brem

E-mail(s)

rbrem@fhcrc.org

Phone

206-667-5659

Fax

206-667-5978

URL

http://www.fhcrc.org/labs/kruglyak/index.html

Organization name

Fred Hutchinson Cancer Research Institute

Department

Human Biology

Lab

Kruglyak

Street address

1100 Fairview Ave. N. D4-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE37

BYxwild_40

Data table header descriptions
ID_REF	Spot number from platform GPL118
CH1_MEAN	Median signal at 635 nm
CH1_BKD_MEAN	Median background signal at 635 nm
CH2_MEAN	Median signal at 532 nm
CH2_BKD_MEAN	Median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	700	52	702	85	0.084632	0
1	353	56	416	68	0.383980	0
2	563	54	450	63	-0.239976	0
3	421	48	365	61	-0.139749	0
4	171	42	183	59	0.098325	0
5	324	55	312	64	0.038090	0
6	79	56	98	68		-50
7	523	39	530	57	0.122189	0
8	423	40	456	58	0.210780	0
9	221	40	271	57	0.396977	0
10	307	40	345	58	0.259567	0
11	1412	41	1587	58	0.312715	0
12	1808	42	862	59	-0.981658	0
13	636	43	594	74	-0.034165	0
14	242	42	301	58	0.436312	0
15	1111	51	1028	64	0.018396	0
16	811	53	795	64	0.103029	0
17	559	42	594	59	0.204730	0
18	308	42	336	59	0.213815	0
19	1128	44	3504	61	1.822657	0

Total number of rows: 7085

Table truncated, full table size 212 Kbytes.

Supplementary data files not provided