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Sample GSM1598 Query DataSets for GSM1598
Status Public on May 01, 2002
Title BY-5.4.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 2016 64 2669 71 -0.136208 0
1 1138 60 1082 68 0.364540 0
2 1144 59 1381 64 -0.003320 0
3 1105 60 1767 65 -0.427488 0
4 548 60 652 65 0.009761 0
5 1511 60 1772 63 0.040136 0
6 828 58 1014 62 -0.029863 0
7 1930 61 2509 64 -0.111328 0
8 2010 65 2220 66 0.128992 0
9 990 64 1332 65 -0.176092 0
10 1720 65 2139 66 -0.048649 0
11 8876 68 8952 71 0.264333 0
12 4748 65 10623 69 -0.896045 0
13 3913 62 4224 64 0.164890 0
14 1145 63 1474 63 -0.106777 0
15 6194 78 6737 74 0.152657 0
16 6973 85 7209 81 0.226828 0
17 4073 86 4322 98 0.192934 0
18 2637 106 2294 126 0.499583 0
19 13175 90 14061 95 0.182235 0

Total number of rows: 7085

Table truncated, full table size 210 Kbytes.




Supplementary data files not provided
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