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Sample GSM1596 Query DataSets for GSM1596
Status Public on May 01, 2002
Title BY-5.2.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1732 67 2290 87 -0.065251 0
1 879 66 777 85 0.571179 0
2 742 67 712 84 0.442819 0
3 706 67 1277 85 -0.560800 0
4 413 65 598 85 -0.221175 0
5 1035 63 1252 83 0.072449 0
6 565 63 752 83 -0.075623 0
7 1252 65 1375 82 0.215294 0
8 1047 65 924 80 0.557176 0
9 719 65 774 80 0.253051 0
10 1125 64 1204 79 0.254196 0
11 6874 68 4928 85 0.829602 0
12 3588 72 6028 88 -0.417832 0
13 2136 69 2092 81 0.378321 0
14 648 67 812 80 0.005391 0
15 3524 68 3148 82 0.511442 0
16 4127 73 3971 83 0.399014 0
17 2859 72 2552 87 0.515821 0
18 1422 91 1130 111 0.724053 0
19 9319 84 8196 95 0.527708 0

Total number of rows: 7085

Table truncated, full table size 208 Kbytes.




Supplementary data files not provided
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