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Sample GSM1595 Query DataSets for GSM1595
Status Public on May 01, 2002
Title BY-5.1.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1111 71 1202 116 0.138617 0
1 598 69 596 113 0.332302 0
2 518 70 858 114 -0.530746 0
3 480 70 717 111 -0.362636 0
4 323 72 435 112 -0.162789 0
5 631 73 723 114 0.074880 0
6 2097 68 2464 110 -0.013288 0
7 790 67 1019 106 -0.135562 0
8 718 66 847 105 0.014510 0
9 493 66 555 106 0.128578 0
10 700 75 821 113 0.021164 0
11 4366 77 3452 115 0.563147 0
12 2519 72 2786 109 0.071454 0
13 1359 70 1532 107 0.056348 0
14 452 72 506 106 0.127057 0
15 1736 75 1874 108 0.112624 0
16 2324 77 2255 110 0.268080 0
17 1685 75 1373 105 0.545563 0
18 917 74 882 103 0.314967 0
19 8961 84 4853 107 1.104418 0

Total number of rows: 7085

Table truncated, full table size 211 Kbytes.




Supplementary data files not provided
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