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Sample GSM1594 Query DataSets for GSM1594
Status Public on May 01, 2002
Title BY-4.4.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1221 68 1365 106 0.212348 0
1 775 73 591 110 0.884667 0
2 635 70 998 114 -0.306562 0
3 609 67 1155 108 -0.610663 0
4 371 79 533 112 -0.188619 0
5 889 78 847 109 0.475314 0
6 748 67 825 102 0.252892 0
7 1008 72 1158 103 0.166571 0
8 873 74 864 105 0.413329 0
9 648 84 668 111 0.357251 0
10 930 82 961 117 0.346055 0
11 6230 82 4036 129 0.993289 0
12 2622 84 14427 127 -2.155018 0
13 1838 81 1848 107 0.352431 0
14 546 83 717 114 -0.041913 0
15 2747 91 2739 124 0.361677 0
16 3464 93 3163 127 0.490238 0
17 2634 90 2286 110 0.564653 0
18 1206 88 836 116 0.974085 0
19 10107 96 7312 121 0.816555 0

Total number of rows: 7085

Table truncated, full table size 209 Kbytes.




Supplementary data files not provided
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