|
| Status |
Public on Jan 27, 2015 |
| Title |
A549 |
| Sample type |
RNA |
| |
|
| Source name |
cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: lung adenocarcinoma epithelial cell
|
| Treatment protocol |
none
|
| Growth protocol |
Cells were cultured on DMEM (PanEco, Russia) supplemented with 10% fetal calf serum (HyClone, USA), 100 mcg/ml penicillin (Sigma, USA), 100 U/ml streptomycin (Sigma, USA) and 2mM L-glutamine (Sigma, USA) at 37°C and 5% CO2. (11) Jurkat cells were maintained in RPMI-1640 medium (PanEco, Russia) with the same supplements. The cells were grown in 25 cm2 or 75 cm2 culture flask (Greiner, Germany) and passaged for every 72 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from samples with Trizol reagent according to manufacture instruction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina® TotalPrep™ RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
RNA was quantified using Nanodrop and quality was checked by Agilent Total RNA 6000 chip. 400 ng of total RNA was amplified by Illumina® TotalPrep™ RNA Amplification Kit (Ambion). Amplified RNA was hybridized with HumanHT-12 gene expression array (Illumina) according to Illumina protocol.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
A549
|
| Data processing |
Data aquisition and analysis were done by GenomeStudio software (Illumina) using gene expression module (GSGX v01.09.2000). Quantile normalization performed with 11 independent gene expression datasets including normalization with data already present in GEO (indicated with GSM ID number). The normalized data can be found on the series record and contain the quantile-normalized signals.
|
| |
|
| Submission date |
Jan 26, 2015 |
| Last update date |
Jan 27, 2015 |
| Contact name |
Larisa Venkova |
| E-mail(s) |
venkovals@gmail.com
|
| Organization name |
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
|
| Lab |
Laboratory for Genomic Analysis of Cell Siganaling
|
| Street address |
Miklukho-Maklaya, 16/10
|
| City |
Moscow |
| ZIP/Postal code |
117997 |
| Country |
Russia |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE65314 |
Experimental gene expression analysis of 12 cancer cell lines |
|
