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Sample GSM1590

Query DataSets for GSM1590

Status

Public on May 01, 2002

Title

5.1.d-BY

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =

Submission date

Apr 26, 2002

Last update date

May 28, 2005

Contact name

Rachel Brem

E-mail(s)

rbrem@fhcrc.org

Phone

206-667-5659

Fax

206-667-5978

URL

http://www.fhcrc.org/labs/kruglyak/index.html

Organization name

Fred Hutchinson Cancer Research Institute

Department

Human Biology

Lab

Kruglyak

Street address

1100 Fairview Ave. N. D4-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE37

BYxwild_40

Data table header descriptions
ID_REF	Spot number from platform GPL118
CH1_MEAN	Median signal at 635 nm
CH1_BKD_MEAN	Median background signal at 635 nm
CH2_MEAN	Median signal at 532 nm
CH2_BKD_MEAN	Median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	930	52	1653	56	0.539284	0
1	394	53	939	58	1.045583	0
2	810	57	818	60	-0.314239	0
3	720	59	962	61	0.123090	0
4	414	54	585	56	0.231484	0
5	791	55	1193	58	0.301127	0
6	211	58	233	62		-50
7	1182	58	1877	65	0.365154	0
8	824	59	1702	65	0.773735	0
9	613	57	1119	62	0.603031	0
10	792	61	1394	74	0.528807	0
11	1107	97	2287	129	0.771552	0
12	3435	59	4983	73	0.216621	0
13	1724	61	3207	71	0.591350	0
14	554	60	957	65	0.528745	0
15	2274	67	4191	80	0.573615	0
16	3609	67	6342	83	0.497581	0
17	2594	66	5209	81	0.696613	0
18	1229	66	2188	79	0.534921	0
19	2069	66	8192	109	1.688941	0

Total number of rows: 7085

Table truncated, full table size 209 Kbytes.

Supplementary data files not provided