|
Status |
Public on Jul 06, 2015 |
Title |
erh020_+IV |
Sample type |
RNA |
|
|
Source name |
+IV_lung
|
Organism |
Mus musculus |
Characteristics |
strain: BALB/c infected with: Influenza A virus 6:2 HK HA +IV tissue: lung
|
Growth protocol |
BALB/c mice were infected with Influenza A virus 6:2 (PR8 backbone) HK wt, 6:2 HK HA +II and 6:2 HK HA +IV. PBS inoculated mice served as control
|
Extracted molecule |
total RNA |
Extraction protocol |
On d2 after Infection, lungs were collected in Trizol. RNA isolation was done using Trizol method and PureLink RNA Mini kit (Ambion)
|
Label |
Biotin
|
Label protocol |
RNA was amplified using the NuGEN Ovation Pico WTA V2 system and then labeled with the NuGEN Encore Biotin Module.
|
|
|
Hybridization protocol |
Labeled targets were hybridized on the mogene2st arry and processed using the Affymetrix system.
|
Scan protocol |
Scanning uses the Affymetrix scanner
|
Description |
erh020
|
Data processing |
The data were analyzed with Partek Genomics Suite 6.6 by the RMA method.
|
|
|
Submission date |
Jan 18, 2015 |
Last update date |
Jul 07, 2015 |
Contact name |
David Finkelstein |
E-mail(s) |
david.finkelstein@stjude.org
|
Phone |
9014953931
|
Organization name |
St Jude Children's Research Hospital
|
Department |
Computational Biology
|
Street address |
332 N. Lauderdale St.
|
City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL16570 |
Series (1) |
GSE65075 |
Acute lung injury results from innate sensing of viruses by an ER stress pathway |
|