|
| Status |
Public on Jul 06, 2015 |
| Title |
erh006_wt |
| Sample type |
RNA |
| |
|
| Source name |
wt_lung
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
infected with: Influenza A virus 6:2 (PR8 backbone) HK wt
tissue: lung
|
| Growth protocol |
BALB/c mice were infected with Influenza A virus 6:2 (PR8 backbone) HK wt, 6:2 HK HA +II and 6:2 HK HA +IV. PBS inoculated mice served as control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
On d2 after Infection, lungs were collected in Trizol. RNA isolation was done using Trizol method and PureLink RNA Mini kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
RNA was amplified using the NuGEN Ovation Pico WTA V2 system and then labeled with the NuGEN Encore Biotin Module.
|
| |
|
| Hybridization protocol |
Labeled targets were hybridized on the mogene2st arry and processed using the Affymetrix system.
|
| Scan protocol |
Scanning uses the Affymetrix scanner
|
| Description |
erh006
|
| Data processing |
The data were analyzed with Partek Genomics Suite 6.6 by the RMA method.
|
| |
|
| Submission date |
Jan 18, 2015 |
| Last update date |
Jul 07, 2015 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE65075 |
Acute lung injury results from innate sensing of viruses by an ER stress pathway |
|
