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Sample GSM1586 Query DataSets for GSM1586
Status Public on May 01, 2002
Title BY-4.1.c
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1509 71 1486 122 0.033770 0
1 994 77 970 127 0.078939 0
2 836 68 849 118 0.028785 0
3 693 69 853 118 -0.278648 0
4 363 66 385 115 0.095054 0
5 1161 67 1069 117 0.158129 0
6 1525 67 1608 120 -0.071834 0
7 1155 64 1141 115 0.046170 0
8 941 64 945 114 0.035278 0
9 694 65 721 117 0.016062 0
10 985 65 967 118 0.073419 0
11 8486 66 8474 117 -0.031615 0
12 3723 65 2811 120 0.400463 0
13 2620 66 2269 116 0.203960 0
14 757 65 776 112 0.017139 0
15 4152 81 3736 155 0.142571 0
16 4231 89 3697 174 0.191073 0
17 3104 73 2920 113 0.068315 0
18 1664 73 1355 109 0.310180 0
19 11360 69 5956 146 0.916113 0

Total number of rows: 7085

Table truncated, full table size 212 Kbytes.




Supplementary data files not provided
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