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Sample GSM1583

Query DataSets for GSM1583

Status

Public on May 01, 2002

Title

4.3.d-BY

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =

Submission date

Apr 26, 2002

Last update date

May 28, 2005

Contact name

Rachel Brem

E-mail(s)

rbrem@fhcrc.org

Phone

206-667-5659

Fax

206-667-5978

URL

http://www.fhcrc.org/labs/kruglyak/index.html

Organization name

Fred Hutchinson Cancer Research Institute

Department

Human Biology

Lab

Kruglyak

Street address

1100 Fairview Ave. N. D4-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE37

BYxwild_40

Data table header descriptions
ID_REF	Spot number from platform GPL118
CH1_MEAN	Median signal at 635 nm
CH1_BKD_MEAN	Median background signal at 635 nm
CH2_MEAN	Median signal at 532 nm
CH2_BKD_MEAN	Median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	1099	58	949	49	-0.269143	0
1	500	56	524	48	0.041233	0
2	725	56	591	48	-0.360223	0
3	704	57	659	48	-0.141763	0
4	334	57	343	49	0.026761	0
5	481	57	595	48	0.308307	0
6	149	55	160	47	0.206421	0
7	1536	57	1404	58	-0.195113	0
8	1136	70	1350	68	0.207019	0
9	650	70	805	63	0.296197	0
10	1246	61	1481	53	0.209940	0
11	6696	64	6164	54	-0.177441	0
12	3681	77	4563	88	0.253119	0
13	3319	81	3205	99	-0.119215	0
14	1027	93	993	135	-0.181614	0
15	6242	92	5986	131	-0.130087	0
16	5517	94	5109	130	-0.182405	0
17	3033	66	3449	60	0.132685	0
18	2018	65	2249	57	0.107386	0
19	2251	73	4580	74	0.989674	0

Total number of rows: 7085

Table truncated, full table size 208 Kbytes.

Supplementary data files not provided