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Sample GSM1581 Query DataSets for GSM1581
Status Public on May 01, 2002
Title 4.1.c-BY
Sample type RNA
 
Channel 1
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 522 42 422 52 -0.354183 0
1 457 40 345 42 -0.439404 0
2 404 55 290 51 -0.524891 0
3 629 58 451 57 -0.513969 0
4 231 51 216 49 -0.086823 0
5 534 78 416 65 -0.356237 0
6 2177 42 1065 40 -1.037286 0
7 456 51 383 42 -0.226824 0
8 371 47 386 43 0.103540 0
9 272 39 222 41 -0.343015 0
10 348 36 273 39 -0.393712 0
11 2279 33 1504 37 -0.593163 0
12 925 34 690 36 -0.424809 0
13 551 37 464 37 -0.246207 0
14 291 36 231 37 -0.373115 0
15 994 35 771 38 -0.366392 0
16 1262 37 953 38 -0.399612 0
17 1097 37 854 39 -0.357867 0
18 368 36 368 39 0.008230 0
19 657 44 1256 41 1.008323 0

Total number of rows: 7085

Table truncated, full table size 208 Kbytes.




Supplementary data files not provided
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