|
| Status |
Public on Jan 08, 2015 |
| Title |
1473_liver |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
gender: male
age: 16 weeks
strain: NZB/BlNJ
|
| Growth protocol |
16 week mice maintained on 12 hr light dark cycle fed chow diet for 8 weeks followed by high-fat/high-sucrose diet for 8 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiazol with homogenization followed by Rneasy spin columns with option Dnase per manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
All target labeling reagents were purchased from Affymetrix (Santa Clara, CA). Double-stranded cDNAs were synthesized from 1ug total RNA through reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter and double strand conversion using the cDNA Synthesis System. Biotin-labeled cRNA was generated from the cDNA and used to probe Affymetrix Mouse Genome HT_MG-430A arrays.
|
| |
|
| Hybridization protocol |
The HT_MG-430A Array plate consists of 96 single MG-430A arrays arranged into standard SBS 96 well plate format. All cDNA and cRNA target preparation steps were processed on a Caliper GeneChip Array Station from Affymetrix. Array hybridization, washing and scanning were performed according to the manufacturer’s recommendations.
|
| Scan protocol |
Scanned images were subjected to visual inspection and a chip quality report was generated by the Affymetrix's GeneChip Operating System (GCOS) and Expression console (Affymetrix).
|
| Data processing |
The image data was processed using the Affymetrix GCOS algorithm utilizing quantile normalization or the Robust Multiarray method (RMA) to determine the specific hybridizing signal for each gene.
|
| |
|
| Submission date |
Jan 07, 2015 |
| Last update date |
Jan 08, 2015 |
| Contact name |
Calvin Pan |
| Organization name |
UCLA
|
| Street address |
675 Charles Young Dr South
|
| City |
Los Angeles |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL8759 |
| Series (2) |
| GSE64769 |
Liver Profiling In HMDP (high-fat/high-sucrose diet) |
| GSE64770 |
Expression Profiling In HMDP (high-fat/high-sucrose diet) |
|
