NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1579 Query DataSets for GSM1579
Status Public on May 01, 2002
Title 3.4.d-BY
Sample type RNA
 
Channel 1
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1560 56 1215 126 -0.218258 0
1 453 51 868 132 1.120053 0
2 2751 53 592 130 -2.298383 0
3 1418 55 837 121 -0.681211 0
4 594 52 442 116 -0.485878 0
5 572 51 660 115 0.312516 0
6 1213 53 981 115 -0.174143 0
7 524 50 1008 124 1.146702 0
8 513 49 937 123 1.058447 0
9 711 49 715 118 0.098443 0
10 989 53 950 121 0.072406 0
11 1987 56 4505 124 1.429455 0
12 7670 69 2011 141 -1.775608 0
13 1433 78 1302 152 0.010884 0
14 525 92 693 157 0.555409 0
15 2070 87 2203 143 0.302503 0
16 1726 90 1938 148 0.377330 0
17 2135 57 2316 135 0.317337 0
18 608 54 1074 152 0.982424 0
19 1169 55 2685 159 1.428648 0

Total number of rows: 7085

Table truncated, full table size 218 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap