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Sample GSM1576 Query DataSets for GSM1576
Status Public on May 01, 2002
Title BY-3.1.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1152 55 1048 117 -0.075216 0
1 705 58 632 118 0.020071 0
2 643 54 622 114 -0.098487 0
3 678 54 856 113 -0.563743 0
4 288 53 311 109 -0.093621 0
5 859 52 819 106 -0.133260 0
6 368 52 436 106 -0.374468 0
7 1124 53 993 106 -0.039974 0
8 1164 52 932 105 0.115271 0
9 477 53 486 106 -0.153862 0
10 1003 55 858 107 0.024148 0
11 6092 57 3931 110 0.347477 0
12 3315 57 7009 111 -1.394118 0
13 2003 53 1668 105 0.007230 0
14 628 55 644 107 -0.218313 0
15 3372 60 2853 111 -0.039452 0
16 3633 62 3017 111 -0.014633 0
17 1669 58 1387 107 0.019886 0
18 1314 57 752 106 0.648452 0
19 12687 61 6036 106 0.778367 0

Total number of rows: 7085

Table truncated, full table size 213 Kbytes.




Supplementary data files not provided
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