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Sample GSM1573 Query DataSets for GSM1573
Status Public on May 01, 2002
Title 3.3.d-BY
Sample type RNA
 
Channel 1
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 539 52 587 131 0.028677 0
1 411 53 493 136 0.119530 0
2 322 57 404 150 0.062401 0
3 504 59 578 154 0.053824 0
4 251 54 376 140 0.384156 0
5 402 51 444 131 -0.041743 0
6 320 49 378 121 0.047041 0
7 483 49 547 137 0.041494 0
8 442 50 509 140 0.036332 0
9 356 52 470 126 0.301902 0
10 442 56 544 136 0.203533 0
11 2831 70 2274 144 -0.250772 0
12 2437 75 1043 133 -1.252505 0
13 691 90 616 148 -0.237291 0
14 377 125 350 178 -0.427450 0
15 1738 80 1317 159 -0.394244 0
16 1692 55 1400 138 -0.251777 0
17 1536 54 1243 131 -0.290824 0
18 586 59 664 133 0.134474 0
19 1014 59 1552 136 0.691814 0

Total number of rows: 7085

Table truncated, full table size 217 Kbytes.




Supplementary data files not provided
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