|
| Status |
Public on Jun 02, 2015 |
| Title |
MN_DLT |
| Sample type |
RNA |
| |
|
| Source name |
human urine sediments
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: urine sediments
gender: male
age: 30
ethnicity: Han
disease state: MN
|
| Treatment protocol |
[collection protocol] A whole-stream early-morning urine specimen was collected into sterile tubes before renal biopsy and processed within 3 hours.
Urine sample was centrifuged at 3,000 g for 30 min and at 13,000 g for 5 min at 4 °C. Urinary cell pellet was lysed by TRIzol® Reagent (Invitrogen™). Specimens were then stored at –80 °C until use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
About 1ug total RNA were polyA tailed and labled with FlashTag™ Biotin HSR Labeling Kit. This kit can be used to label any RNA sample, including total RNA, severely degraded RNA, plant RNA, and low molecular weight RNA.
|
| |
|
| Hybridization protocol |
The labled RNA were hybridized for 16 hr at 48C on Affymetrix miRNA Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
| Description |
MicroRNA expression data from unrine sediments
|
| Data processing |
The data were analyzed with miRNA QCTool using Affymetrix default analysis settings and quantile as normalization method.
|
| |
|
| Submission date |
Dec 18, 2014 |
| Last update date |
Jun 02, 2015 |
| Contact name |
Nannan Wang |
| E-mail(s) |
plumppumpkin@163.com
|
| Organization name |
Chinese PLA General Hospital, Chinese PLA Institute of Nephrology,National Clinical Research Center for Kidney Diseases
|
| Department |
Department of Nephrology
|
| Lab |
State Key Laboratory of Kidney Diseases
|
| Street address |
Fuxing Road NO.28
|
| City |
Beijing |
| ZIP/Postal code |
100853 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE64306 |
MicroRNA profiling by array of human urinary sediments from IgAN patients |
|
