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Sample GSM1568 Query DataSets for GSM1568
Status Public on May 01, 2002
Title BY-2.4.a
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1944 33 2342 62 -0.049671 0
1 1092 35 854 67 0.630574 0
2 1065 35 6044 69 -2.331260 0
3 1082 35 1852 66 -0.565436 0
4 477 36 492 65 0.251577 0
5 1275 35 1127 62 0.424521 0
6 390 34 562 58 -0.296512 0
7 1894 35 1680 59 0.402677 0
8 1682 36 1396 59 0.504999 0
9 730 36 679 59 0.367702 0
10 1614 45 1250 60 0.603918 0
11 5905 50 4587 61 0.576467 0
12 3061 43 6811 59 -0.956688 0
13 2784 44 2944 59 0.130639 0
14 845 45 977 59 0.006540 0
15 4456 48 5130 61 0.003458 0
16 5048 52 5111 65 0.190668 0
17 2331 46 2185 61 0.310445 0
18 1751 45 1201 59 0.784090 0
19 9420 41 3247 60 1.762270 0

Total number of rows: 7085

Table truncated, full table size 205 Kbytes.




Supplementary data files not provided
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