|
| Status |
Public on Mar 01, 2007 |
| Title |
HELA cells stimulated with EGF for 240 minutes |
| Sample type |
RNA |
| |
|
| Source name |
Human cervical carcinoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
HeLa cells
|
| Treatment protocol |
HELA cells were grown in DMEM supplemented with 10% FCS and antibiotics. Cells were serum deprived for 24 hours. Following stimulation with 10 ng/ml EGF for 240 minutes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U!33A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using laser confocal scanner.
|
| Description |
Gene expression data from unstimulated HELA cells.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jan 18, 2007 |
| Last update date |
Mar 01, 2007 |
| Contact name |
Tal Shay |
| Organization name |
Weizmann Institute of Science
|
| Department |
Physics of Complex Systems
|
| Street address |
Herzel
|
| City |
Rehovot |
| ZIP/Postal code |
26100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE6783 |
Expression data from HELA cells subject to EGF stimulation |
| GSE6786 |
HeLa cells and MCF10A cells subject to EGF stimulation |
|
