|
| Status |
Public on Dec 15, 2010 |
| Title |
MH-S_IL-4_rep01 |
| Sample type |
RNA |
| |
|
| Source name |
ATCC_CRL-2019, IL-4 treated
|
| Organism |
Mus musculus |
| Characteristics |
strain:BALB/c Mouse', 'cell type:alveolar macrophage', 'source: cell culture', 'condition: IL-4 treated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
trizol extraction followed by qiagen RNeasy total RNA cleanup protocol
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures using affy GCS3000
|
| Description |
Intensity of hybridization for each probe pair was computed by GCOS 1.2 software (Affymetrix). (For more detailed methods, please refer to the website of the Malaria Research Institute Gene Array Core Facility (MRI-GACF) at the Johns Hopkins Bloomberg School of Public Health http://jhmmi.jhsph.edu)
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Jan 18, 2007 |
| Last update date |
Dec 15, 2010 |
| Contact name |
Joshua J Reece |
| E-mail(s) |
josh.reece@gmail.com
|
| Organization name |
Johns Hopkins University BSPH
|
| Department |
MMI
|
| Lab |
Lab of Alan Scott
|
| Street address |
615 N. Wolfe Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6785 |
Activation and Function of Alternatively and Classically Activated MH-S Alveolar Macrophages |
|
