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Sample GSM1567 Query DataSets for GSM1567
Status Public on May 01, 2002
Title BY-2.3.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1218 58 1311 137 -0.079012 0
1 660 56 713 131 -0.008175 0
2 618 55 762 129 -0.230775 0
3 700 55 930 128 -0.376008 0
4 271 55 344 126 -0.075001 0
5 855 58 888 125 0.001192 0
6 144 53 220 120 -50
7 1195 53 1063 121 0.216059 0
8 1136 54 946 120 0.327782 0
9 450 54 509 120 -0.035974 0
10 949 55 805 121 0.324574 0
11 4789 56 4464 120 0.062026 0
12 2886 56 4051 119 -0.536166 0
13 1693 56 1681 119 0.005955 0
14 549 56 700 119 -0.298655 0
15 2824 59 2693 123 0.043807 0
16 2830 59 2589 122 0.105944 0
17 1832 57 1676 117 0.125493 0
18 1054 58 960 116 0.177198 0
19 9480 59 2458 114 1.945203 0

Total number of rows: 7085

Table truncated, full table size 210 Kbytes.




Supplementary data files not provided
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