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Sample GSM1561

Query DataSets for GSM1561

Status

Public on May 01, 2002

Title

2.2.d-BY

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =

Submission date

Apr 26, 2002

Last update date

May 28, 2005

Contact name

Rachel Brem

E-mail(s)

rbrem@fhcrc.org

Phone

206-667-5659

Fax

206-667-5978

URL

http://www.fhcrc.org/labs/kruglyak/index.html

Organization name

Fred Hutchinson Cancer Research Institute

Department

Human Biology

Lab

Kruglyak

Street address

1100 Fairview Ave. N. D4-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE37

BYxwild_40

Data table header descriptions
ID_REF	Spot number from platform GPL118
CH1_MEAN	Median signal at 635 nm
CH1_BKD_MEAN	Median background signal at 635 nm
CH2_MEAN	Median signal at 532 nm
CH2_BKD_MEAN	Median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	354	54	264	45	-0.615652	0
1	180	53	184	43	-0.010754	0
2	158	54	180	43	0.235972	0
3	305	53	228	44	-0.615339	0
4	142	54	138	44	-0.066463	0
5	211	54	196	44	-0.208314	0
6	245	52	247	43	-0.081652	0
7	237	54	210	45	-0.310998	0
8	162	54	222	45	0.551097	0
9	180	53	187	45	-0.000558	0
10	189	52	258	45	0.475057	0
11	1271	53	1284	46	-0.138124	0
12	1453	54	520	46	-1.723058	0
13	379	55	348	46	-0.263066	0
14	237	56	224	49	-0.210255	0
15	686	55	564	48	-0.451890	0
16	793	60	671	53	-0.407827	0
17	638	60	603	53	-0.233259	0
18	278	55	361	48	0.327498	0
19	1027	56	1521	49	0.438614	0

Total number of rows: 7085

Table truncated, full table size 207 Kbytes.

Supplementary data files not provided