|
| Status |
Public on Apr 30, 2015 |
| Title |
lung, nontumor adjacent_18974 |
| Sample type |
RNA |
| |
|
| Source name |
lung, nontumor adjacent
|
| Organism |
Homo sapiens |
| Characteristics |
patient_id1: 18974N
patient_id: 18974
Sex: Male
race: European American
age: 55
disease state: non-small cell lung cancer (NSCLC)
histology: normal
smoking status: ever
smoking packyears: 39
death due to cancer: yes
survival after surgery (days): 1180
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was extracted from tumor and matching adjacent non-tumor lung using miRNA Kit (QIAGEN). RNA quality was determined using Agilent Bioanalyzer. RNA was hybridized to Illumina HumanRef-8 v3 Expression Beadchip arrays.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina Protocol Illumina TotalPrep-96 RNA Amplification Kit (Life Technologies)
|
| |
|
| Hybridization protocol |
Labeled cRNA was hybridised to Illumina HumanRefseq8 v3
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
nontumor adjacent
|
| Data processing |
Genome Studio v2010.3
|
| |
|
| Submission date |
Nov 19, 2014 |
| Last update date |
Apr 30, 2015 |
| Contact name |
Ana I Robles |
| E-mail(s) |
Ana_Robles@nih.gov
|
| Phone |
301-496-1729
|
| Organization name |
National Cancer Institute/NIH
|
| Lab |
Laboratory of Human Carcinogenesis
|
| Street address |
37 Convent Dr 37/3060D
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE63459 |
Multi-platform Characterization of Stage I Lung Adenocarcinoma (mRNA expression) |
|
