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| Status |
Public on Mar 31, 2015 |
| Title |
IEL-ab |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
90852 |
| Tag Length |
10 |
| |
|
| Source name |
Intestinal TCRαβ IEL
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Intestinal TCRαβ IEL
strain: C57BL/6
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| Treatment protocol |
IEL were isolated from murine gut as described in Shires et al (2001) Immunity, 15, 419-34
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with trizol and processed as in the supplied protocol (see also Shires et al (2001), Immunity, 15, 419-34
Complete SAGE protocol provided in Series supplementary file Hayday_SAGE_Protocol_Nov 2001.pdf
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| Data processing |
supplementary files include data normalized to 100,000 tags
Analysis was performed using SAGE300 and eSAGE software
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| Submission date |
Nov 07, 2014 |
| Last update date |
Mar 31, 2015 |
| Contact name |
Lucie Abeler-Dörner |
| Organization name |
King's College London
|
| Department |
Immunobiology
|
| Lab |
Hayday
|
| Street address |
Great Maze Pond
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| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platform ID |
GPL11 |
| Series (1) |
| GSE63090 |
Gene expression of conventional and unconventional T cell subsets |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSM1540961_Hayday_SAGE_library_1.txt.gz |
48.8 Kb |
(ftp)(http) |
TXT |
| Processed data provided as supplementary file |
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