|
| Status |
Public on Dec 11, 2015 |
| Title |
Human MCF-7 cells treated with MELK-T1 during 48 hours 16002 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7
treatment: MELK-T1
time (hours): 48
|
| Treatment protocol |
MCF-7 cells were incubated with 10 µM of MELK-T1 for 48 h. DMSO was used as negative control.
|
| Growth protocol |
MCF-7 cells were cultured in high-glucose DMEM (LifeTechnologies) supplemented with 10% FBS (LifeTechnologies).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed using RLT buffer (Qiagen) and RNA extracted with the RNeasy 96 kit (p/n 74181 Qiagen).
|
| Label |
biotin
|
| Label protocol |
All microarray related steps for target preparation including amplification of total RNA and labeling were carried out as described in the GeneChip®3’ IVT, Express Kit User Manual , (Affymetrix 2004).
|
| |
|
| Hybridization protocol |
Target hybridization was processed on the GeneTitan® Instrument according to the instructions provided in the User Guide for Expression Array Plates (P/N 702933).
|
| Scan protocol |
Plate scan was processed on the GeneTitan® Instrument according to the instructions provided in the User Guide for Expression Array Plates (P/N 702933). Images were analyzed using the GeneChip® Command Console Software (AGCC), (Affymetrix).
|
| Data processing |
The data were analyzed with R version 3.0.1 using RMA algorithm for preprocessing (affy_1.38.1) and Entrez based alternative CDF version 15.1.0 for probeset annotation
|
| |
|
| Submission date |
Oct 18, 2014 |
| Last update date |
Dec 11, 2015 |
| Contact name |
Steven Osselaer |
| E-mail(s) |
sosselae@its.jnj.com
|
| Organization name |
Janssen Pharmaceutica NV
|
| Street address |
Turnhoutseweg 30
|
| City |
Beerse |
| ZIP/Postal code |
2340 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL19309 |
| Series (1) |
| GSE62477 |
MELK-T1, a small molecule inhibitor of protein kinase MELK, decreases DNA damage tolerance in highly proliferating cancer cells |
|
