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Sample GSM151427 Query DataSets for GSM151427
Status Public on Dec 01, 2007
Title Sepsis Patient P5
Sample type RNA
 
Channel 1
Source name Neutrophil
Organism Homo sapiens
Characteristics Sepsis Patient
Infection type: Gram-Positive
Biomaterial provider Nepean Hospital Intensive Care Unit
Extracted molecule total RNA
Extraction protocol RiboPure-Blood kit (AmbionTM)
Label Cy5
Label protocol SuperScriptÖ Plus Indirect cDNA Labeling System for DNA Microarrays (Invitrogen)
 
Channel 2
Source name Cell line
Organism Homo sapiens
Characteristics Universal Human Reference RNA
Biomaterial provider Stratagene
Extracted molecule total RNA
Extraction protocol not applicable
Label Cy3
Label protocol SuperScriptÖ Plus Indirect cDNA Labeling System for DNA Microarrays (Invitrogen)
 
 
Hybridization protocol SuperScriptÖ Plus Indirect cDNA Labeling System for DNA Microarrays (Invitrogen)
Scan protocol GenePix 4000B laser scanner and GenePix software (Axon Instruments)
Description none
Data processing Background subtraction followed by Lowess print-tip group Normalization, include genes which have 1. at least 80% of expression data with 2 fold change in either direction from the gene's median value, (2) at least 50% of expression data non-missing
 
Submission date Dec 14, 2006
Last update date Oct 09, 2007
Contact name Benjamin Man Piu Tang
E-mail(s) benjamin@clubsalsa.com.au
Phone 61415614883
Organization name Nepean Hospital
Department Intensive Care Medicine
Street address 12/29 Cook Street
City Glebe
State/province NSW
ZIP/Postal code 2037
Country Australia
 
Platform ID GPL4274
Series (1)
GSE6535 Critically ill patients with either gram-positive sepsis or gram-negative sepsis

Data table header descriptions
ID_REF
VALUE normalized log (cy5/cy3)

Data table
ID_REF VALUE
AB000464 1.040117741
AB000509 0.00443454
AB000520 2.199834347
AB000895 1.026842594
AB001328 -0.386391312
AB001451 -1.465319633
AB002282 -2.263609171
AB002293 -0.345250309
AB002296 0.622903109
AB002297 -0.094822824
AB002298 -0.214478314
AB002299 -0.029126193
AB002301 1.087119818
AB002304 0.439953268
AB002306 -0.78360486
AB002308 -0.667490661
AB002313 0.115667768
AB002314 -0.672159255
AB002316 -0.848444402
AB002318 -0.374037266

Total number of rows: 18664

Table truncated, full table size 395 Kbytes.




Supplementary file Size Download File type/resource
GSM151427.gpr.gz 2.1 Mb (ftp)(http) GPR

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