|
| Status |
Public on Dec 01, 2007 |
| Title |
Sepsis Patient N25 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Neutrophil
|
| Organism |
Homo sapiens |
| Characteristics |
Sepsis Patient
Infection type: Gram-Negative
|
| Biomaterial provider |
Nepean Hospital Intensive Care Unit
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RiboPure-Blood kit (AmbionTM)
|
| Label |
Cy5
|
| Label protocol |
SuperScriptÖ Plus Indirect cDNA Labeling System for DNA Microarrays (Invitrogen)
|
| |
|
| Channel 2 |
| Source name |
Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
Universal Human Reference RNA
|
| Biomaterial provider |
Stratagene
|
| Extracted molecule |
total RNA |
| Extraction protocol |
not applicable
|
| Label |
Cy3
|
| Label protocol |
SuperScriptÖ Plus Indirect cDNA Labeling System for DNA Microarrays (Invitrogen)
|
| |
|
| |
| Hybridization protocol |
SuperScriptÖ Plus Indirect cDNA Labeling System for DNA Microarrays (Invitrogen)
|
| Scan protocol |
GenePix 4000B laser scanner and GenePix software (Axon Instruments)
|
| Description |
none
|
| Data processing |
Background subtraction followed by Lowess print-tip group Normalization, include genes which have 1. at least 80% of expression data with 2 fold change in either direction from the gene's median value, (2) at least 50% of expression data non-missing
|
| |
|
| Submission date |
Dec 14, 2006 |
| Last update date |
Oct 09, 2007 |
| Contact name |
Benjamin Man Piu Tang |
| E-mail(s) |
benjamin@clubsalsa.com.au
|
| Phone |
61415614883
|
| Organization name |
Nepean Hospital
|
| Department |
Intensive Care Medicine
|
| Street address |
12/29 Cook Street
|
| City |
Glebe |
| State/province |
NSW |
| ZIP/Postal code |
2037 |
| Country |
Australia |
| |
|
| Platform ID |
GPL4274 |
| Series (1) |
| GSE6535 |
Critically ill patients with either gram-positive sepsis or gram-negative sepsis |
|
