|
| Status |
Public on Jul 06, 2007 |
| Title |
SN:1022907;Fetal Cartilage, biological rep2 validation |
| Sample type |
RNA |
| |
|
| Source name |
RNA isolated from Fetal Cartilage 18-22 weeks - rep2 validation
|
| Organism |
Homo sapiens |
| Characteristics |
Normal Fetal Cartilage from Distal Femur
|
| Treatment protocol |
Distal fetal cartilage from femur of Homo sapiens at 18-22 weeks was dissected from non cartilage tissue using a dissecting scope. RNA was isolated from Cartilage using the Trizol solution (GibcoBRL).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip U133 plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Normal human fetal cartilage from 18-22 weeks.
|
| Data processing |
The data were analyzed using R 2.2.1 and RMA as normalization method with PM model.
|
| |
|
| Submission date |
Dec 11, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Vincent Anthony Funari |
| E-mail(s) |
vincent.funari@cshs.org
|
| Phone |
3104980389
|
| Organization name |
Cedars Sinai
|
| Department |
Medical Genetics
|
| Street address |
8700 Beverly Drive
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90048 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE6565 |
Fetal cartilage selective genes identified in a genome-scale analysis |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
