|
| Status |
Public on Nov 06, 2015 |
| Title |
PDAC_15 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
Fresh frozen tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: pancreatic ductal adenocarcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cell pellets using mirVana kit (Ambion/Life Technologies) according to the manufacturer’s instructions. Total RNA was eluted in 100 µl H2O, concentration measured on NanoDrop ND-1000.
|
| Label |
Cy3
|
| Label protocol |
miRNA was labeled with Cy-3 according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies, using 200 ng total RNA as input.
|
| |
|
| Hybridization protocol |
miRNA microarrays Cy-3-labeled cRNA was hybridized according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies.
|
| Scan protocol |
The slides were scanned immediately after washing on the Agilent Microarray Scanner G2565C using protocol GE1_107_Sep09 for the expression microarrays and protocol.
|
| Description |
Gene expression of fresh frozen pancreatic ductal adenocarcinoma patient 15
|
| Data processing |
The scanned images were analyzed using Feature Extraction Software 10.7.3.1 (Agilent Technologies) using default parameters. The expression profiles was RMA normalized for miRNA expression data. Filtering of controls, unannotated genes and low expressed probes was done.
|
| |
|
| Submission date |
Sep 02, 2014 |
| Last update date |
Nov 06, 2015 |
| Contact name |
Vandana Sandhu |
| E-mail(s) |
vansan@rr-research.no
|
| Organization name |
Radiumhospitalet, Oslo
|
| Department |
Department of Genetics
|
| Street address |
Ullernchausseen 70
|
| City |
Oslo |
| State/province |
oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL15159 |
| Series (2) |
| GSE60978 |
mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [miRNA] |
| GSE60980 |
mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. |
|
