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Sample GSM1489258 Query DataSets for GSM1489258
Status Public on Jun 29, 2016
Title 610B1-HPC
Sample type genomic
Source name human PSC-derived hematopoietic precursor cells
Organism Homo sapiens
Characteristics cell type: Pluripotent-stem cell derived hematopoietic precursor cells
Growth protocol We differeniated human pluripotent stem cell lines by using StemPro-34 (Invitrogen) based media for 15 days.Cytokines or a chemical compound were utilized as follows during differentiation: on day 0-1, human BMP4 (10 ng/ml) and Rock-inhibitor (Y27632) (10 μM); on day 1-4, human BMP4 (10 ng/ml) and human basic fibroblast growth factor (bFGF; 5 ng/ml); on day 4-8, human vascular endothelial growth factor (hVEGF; 10 ng/mL), hbFGF (1 ng/mL), human interleukin-6 (hIL-6; 10 ng/mL), hIL-3 (40 ng/mL), hIL-11 (5 ng/mL), human stem cell factor (hSCF; 100 ng/mL), and human Fms-like tyrosine kinase 3 ligand (hFlt3L; 100 ng/mL); and on Day 8-15, hVEGF (10 ng/mL), hIL-6 (10 ng/mL), hIL-3 (40 ng/mL), hIL-11 (5 ng/mL), hSCF (100 ng/mL), hFlt3L (100 ng/mL), human erythropoietin (hEPO; 4U/ml), and human thrombopoietin (50 ng/mL)
Extracted molecule genomic DNA
Extraction protocol GenomicDNA lysates were extracted with DNeasy(R) blood and tissue kit (QIAgen) in accordance with manufacture's protocol.
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Standard Illumina protocol
Scan protocol Standard recommended Illumina scanner setting protocol using BeadArray Reader
Description SAMPLE 9
Data processing Illumina GenomeStudio Software
Submission date Aug 27, 2014
Last update date Jun 29, 2016
Contact name Yoshinori Yoshida
Organization name Kyoto University
Street address 53, Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL13534
Series (2)
GSE60811 Reprogramming-associated aberrant DNA methylation determines hematopoietic differentiation capacity of human induced pluripotent stem cells [PSCderived_HPCs_methylation]
GSE60924 Reprogramming-associated aberrant DNA methylation determines hematopoietic differentiation capacity of human induced pluripotent stem cells

Data table header descriptions
VALUE Average Beta
Detection Pval

Data table
ID_REF VALUE Detection Pval
cg00000029 0.7566146 0
cg00000108 0.9387818 0
cg00000109 0.9018842 0
cg00000165 0.2131148 0
cg00000236 0.8690994 0
cg00000289 0.8071455 0
cg00000292 0.8040792 0
cg00000321 0.4361869 0
cg00000363 0.3301364 0
cg00000622 0.02133638 0
cg00000658 0.8638083 0
cg00000714 0.3104002 0
cg00000721 0.9252431 0
cg00000734 0.1405507 0
cg00000769 0.08553887 0
cg00000807 0.849024 0
cg00000884 0.8671479 0
cg00000905 0.1748252 0
cg00000924 0.5813366 0
cg00000948 0.9230036 0

Total number of rows: 485577

Table truncated, full table size 10941 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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