|
| Status |
Public on Aug 27, 2014 |
| Title |
K8 WT variant female bio replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver from female human wild type K8
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver tissue under basal condition
strain: FVB/N backround
genotype/variation: K8 WT variant
gender: female
|
| Treatment protocol |
Animals are untreated
|
| Growth protocol |
All animals were housed animal facilty of the Ulm University (Ulm, Germany)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from livers were extracted by Qiagen Rneasy mini isolation kit (#74106).
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
2µg of total RNA were used for the One-Cycle target labeling assay, further processed and hybridized to the GeneChip® Mouse 430 Plus 2.0 array from Affymetrix according to manufacturer’s instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
Gene expression
|
| Data processing |
Statistical analyses were carried out using BRB Array Tools (http://linus.nci.nih.gov/BRB-ArrayTools.html). Arrays have been normalized using Robust Multichip Average (RMA)
|
| |
|
| Submission date |
Aug 26, 2014 |
| Last update date |
Aug 27, 2014 |
| Contact name |
Pavel Strnad |
| E-mail(s) |
pstrnad@ukaachen.de
|
| Organization name |
RWTH Aachen University
|
| Street address |
Pauwelsstraße 30
|
| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE60780 |
Gene expression profiling in mice overexpressing human wild type (WT) keratin 8 (K8) or K8 variants (G62C and R341H). |
|
