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Status |
Public on Aug 27, 2014 |
Title |
K8 G62C variant_female bio replicate 3 |
Sample type |
RNA |
|
|
Source name |
Liver from female human K8 G62C variant
|
Organism |
Mus musculus |
Characteristics |
tissue: liver tissue under basal condition strain: FVB/N backround genotype/variation: K8 G62C variant gender: female
|
Treatment protocol |
Animals are untreated
|
Growth protocol |
All animals were housed animal facilty of the Ulm University (Ulm, Germany)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA from livers were extracted by Qiagen Rneasy mini isolation kit (#74106).
|
Label |
biotin
|
Label protocol |
As per manufacturer (Affymetrix)
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|
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Hybridization protocol |
2µg of total RNA were used for the One-Cycle target labeling assay, further processed and hybridized to the GeneChip® Mouse 430 Plus 2.0 array from Affymetrix according to manufacturer’s instructions.
|
Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
Description |
Gene expression
|
Data processing |
Statistical analyses were carried out using BRB Array Tools (http://linus.nci.nih.gov/BRB-ArrayTools.html). Arrays have been normalized using Robust Multichip Average (RMA)
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|
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Submission date |
Aug 26, 2014 |
Last update date |
Aug 27, 2014 |
Contact name |
Pavel Strnad |
E-mail(s) |
pstrnad@ukaachen.de
|
Organization name |
RWTH Aachen University
|
Street address |
Pauwelsstraße 30
|
City |
Aachen |
ZIP/Postal code |
52074 |
Country |
Germany |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE60780 |
Gene expression profiling in mice overexpressing human wild type (WT) keratin 8 (K8) or K8 variants (G62C and R341H). |
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