|
| Status |
Public on Dec 07, 2007 |
| Title |
adult head_AA22_experimentcnbwtemp |
| Sample type |
RNA |
| |
|
| Source name |
adult head
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cn bw stock
|
| Biomaterial provider |
Young Laboratory
|
| Treatment protocol |
25 C DD (after 12-hr 18 C/12-hr 25 C thermocycle in constant darkness)
|
| Growth protocol |
initially 25 C DD, then 12-hr 18 C/12-hr 25 C thermocycle in constant darkness
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Flies were immediately frozen on dry ice. Approximately 100-200 heads were isolated on sieves after breaking the frozen flies
|
| Label |
biotin
|
| Label protocol |
Approximately 25 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
none
|
| Data processing |
Bioconductor Robust Multi-array Average
|
| |
|
| Submission date |
Dec 06, 2006 |
| Last update date |
Dec 12, 2006 |
| Contact name |
Herman Wijnen |
| E-mail(s) |
hw3a11@soton.ac.uk
|
| Phone |
02380594336
|
| Organization name |
University of Southampton
|
| Department |
Centre for Biological Sciences
|
| Lab |
Wijnen
|
| Street address |
Life Sciences Building 85 - Highfield Campus
|
| City |
Southampton |
| State/province |
Hampshire |
| ZIP/Postal code |
SO17 1BJ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL72 |
| Series (2) |
|
