|
| Status |
Public on Sep 26, 2014 |
| Title |
U937-shEMPTY-Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
U937 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U937
shRNA: empty vector
|
| Treatment protocol |
Two shRNAs in H1 lentiviral vectors targeting the DEK transcript (a kind gift from Dr David Markovitz) and the corresponding empty vector were used to transduce U937 cells. After 48h, successfully transduced cells were sorted based on GFP expression and expanded for 4 days before RNA extraction.
|
| Growth protocol |
U937 cells were grown in RPMI 1640 medium supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Illumina®Totalprep-96RNA Amplification kit 4397949 RevB
|
| |
|
| Hybridization protocol |
Illumina hybridization protocol (Manuel 11322187 Revision A)
|
| Scan protocol |
Standard Illumina iScan N240 scanning protocol
|
| Description |
Exp2_shEMPTY.a1.lbe1
|
| Data processing |
The data were normalised using quantile normalisation with BASE
|
| |
|
| Submission date |
Aug 25, 2014 |
| Last update date |
Sep 26, 2014 |
| Contact name |
Carl Sanden |
| E-mail(s) |
carl.sanden@med.lu.se
|
| Organization name |
Lund University
|
| Department |
Department of Hematology
|
| Street address |
BMC B13, Klinikgatan 26
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE60732 |
Gene expression changes upon knockdown of DEK in the U937 cell line |
|
