|
| Status |
Public on Dec 06, 2007 |
| Title |
A27 |
| Sample type |
RNA |
| |
|
| Source name |
human bone marrow from iliac crest of normal donor
|
| Organism |
Homo sapiens |
| Characteristics |
female, 34 years old
|
| Biomaterial provider |
Hematology of Torvergata Polyclinic (Rome)
|
| Treatment protocol |
none
|
| Growth protocol |
A27 bone marrow aspirate was collected with informed consent. after red blood cells lysis, nucleated cells were counted and 4.7x10(5) cells per square centimeter were plated. cells were cultured in T75 flask (Becton Dickinson) containing 30mL of a growth medium consisting of a-MEM, 2mM L-glutamine, 100U/mL penicillin, 20% fetal bovine serum (FBS, Invitrogen). medium was replaced on day 1 and then twice a week. first passage was performed on days 12-16 when colonies began to merge and cells were replated in T75 flasks at 0.1x10(5) cells/cm(2). (Kuznetsov S.A. et al. JBMR volume 12, number 9, 1997 - "Single-Colony Derived Strains of Human Marrow Stromal Fibroblasts Form Bone After Transplantation In Vivo")
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIZOL (Invitrogen) following the manufacturer's instructions and then purified using RNeasy MicroKit (Qiagen) following manufacturer's recommendations
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix One-Cycle Target Labeling protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix Hybridization Protocol
|
| Scan protocol |
standard Affymetrix Scanner procedure
|
| Description |
none
|
| Data processing |
data in the value column were generated and calculated with GCOS 1.4 (scaled to 120)
|
| |
|
| Submission date |
Dec 06, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Enrico Tagliafico |
| E-mail(s) |
enrico.tagliafico@unimore.it
|
| Organization name |
University of Modena and Reggio Emilia
|
| Department |
Center for Genome Research
|
| Street address |
Via Campi 287
|
| City |
Modena |
| State/province |
MO |
| ZIP/Postal code |
41126 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE6460 |
Human mesenchymal stem cells |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
