|
| Status |
Public on Jun 01, 2015 |
| Title |
HCT15 |
| Sample type |
RNA |
| |
|
| Source name |
HCT15 CRC cell line, standard culture conditions
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT15
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Standard culture conditions for each cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using miRNeasy Mini Kit (Qiagen), according to the manufacturer’s protocol, without the separation step between mRNAs and microRNAs
|
| Label |
Biotin
|
| Label protocol |
Synthesis of cDNA and biotinylated cRNA was performed using the IlluminaTotalPrep RNA Amplification Kit (Ambion), according to the manufacturer’s protocol using 500ng of total RNA
|
| |
|
| Hybridization protocol |
Hybridization of cRNAs (750 ng) was carried out using Illumina Human 48k gene chips (Human HT-12 V4 BeadChip)
|
| Scan protocol |
Hybridized Beadchips were scanned with the Illumina BeadArray Reader
|
| Description |
CRC cell line
|
| Data processing |
Non-normalized data were generated by the Illumina GenomeStudio software version V2011.1 . Data were Loess normalized with the Lumi R package
Signal values and detection p-values as provided by the Illumina Genome Studio software. Normalized signal values as provided by the Lumi R package.
|
| |
|
| Submission date |
Jul 29, 2014 |
| Last update date |
Jun 01, 2015 |
| Contact name |
Enzo Medico |
| E-mail(s) |
enzo.medico@ircc.it
|
| Phone |
+39-011-9933234
|
| Organization name |
Candiolo Cancer Institute, University of Torino
|
| Department |
Oncology
|
| Lab |
Laboratory of Oncogenomics
|
| Street address |
Strada Prov. 142, km 3,95
|
| City |
Candiolo |
| State/province |
TO |
| ZIP/Postal code |
10060 |
| Country |
Italy |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE59857 |
Global gene expression profiling of human colorectal cancer cell lines |
|
