Strain: CEN.PK113-7D (Lab reference strain) transformed with pYX212-NOX plasmid. Chemostat cultivation at the critical dilution rate.
Biomaterial provider
Goutham Vemuri
Treatment protocol
Chemostat cultivation at the critical dilution rate
Growth protocol
Growth on minimal media (Verduyn, (1992) Yeast 8, 501-517) using 10 g/L glucose as the sole carbon source.
Extracted molecule
total RNA
Extraction protocol
The extraction of RNA, cDNA synthesis, cRNA synthesis and labelling, as well as array hybridisation to Affymetrix Yeast Genome 2.0 arrays (Affymetrix, Santa Clara, CA, USA) were performed as described in the Affymetrix manual. Affymetrix (2000) Affymetrix GeneChip Expression Analysis Technical Manual. (Affymetrix, Santa Clara, CA).
Label
biotin
Label protocol
The extraction of RNA, cDNA synthesis, cRNA synthesis and labelling, as well as array hybridisation to Affymetrix Yeast Genome 2.0 arrays (Affymetrix, Santa Clara, CA, USA) were performed as described in the Affymetrix manual. Affymetrix (2000) Affymetrix GeneChip Expression Analysis Technical Manual. (Affymetrix, Santa Clara, CA).
Hybridization protocol
The extraction of RNA, cDNA synthesis, cRNA synthesis and labelling, as well as array hybridisation to Affymetrix Yeast Genome 2.0 arrays (Affymetrix, Santa Clara, CA, USA) were performed as described in the Affymetrix manual. Affymetrix (2000) Affymetrix GeneChip Expression Analysis Technical Manual. (Affymetrix, Santa Clara, CA).
Scan protocol
The extraction of RNA, cDNA synthesis, cRNA synthesis and labelling, as well as array hybridisation to Affymetrix Yeast Genome 2.0 arrays (Affymetrix, Santa Clara, CA, USA) were performed as described in the Affymetrix manual. Affymetrix (2000) Affymetrix GeneChip Expression Analysis Technical Manual. (Affymetrix, Santa Clara, CA).
Description
The extraction of RNA, cDNA synthesis, cRNA synthesis and labelling, as well as array hybridisation to Affymetrix Yeast Genome 2.0 arrays (Affymetrix, Santa Clara, CA, USA) were performed as described in the Affymetrix manual. Affymetrix (2000) Affymetrix GeneChip Expression Analysis Technical Manual. (Affymetrix, Santa Clara, CA).
