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Sample GSM1431375 Query DataSets for GSM1431375
Status Public on Jul 07, 2016
Title DCIS-CMB_354
Sample type RNA
Source name Ductal carcinoma in situ (DCIS)
Organism Homo sapiens
Characteristics tissue type: Ductal carcinoma in situ (DCIS)
er (ihc): NA
pr (ihc): NA
her2 (ihc): NA
grade (combined): 3
grade (elston): NA
grade (eortc): NA
lymph node: 0
age (years): 44
size (mm): NA
pam50: Her2
death event: NA
iPSilateral event: NA
dmfs event: NA
recurrence event: NA
recurrence location: NA
recurrence type: NA
follow-up time: NA
source: Akershus
Extracted molecule total RNA
Extraction protocol Tumor tissues were homogenized using TRIzol Reagent (Invitrogen, Life Technologies, USA), and purified with RNeasy mini columns (Qiagen, Netherlands). The quality of RNA was assessed using NanoDrop ND-1000 Spectrophotometer, version 3.7.1 (NanoDrop Technologies, Thermo Fisher Scientific Inc, USA).
Label Cy3
Label protocol 200ng RNA was used as input for labeling according to manufacturer's protocol.
Hybridization protocol RNA was hybridized to Human miRNA Microarray Release 16.0, 8x60K (G4872A-031181) according to manufacturer's instructions.
Scan protocol Feature extraction was performed using Agilent Scanner G2565A and Feature Extraction software version
Description miRNA profile of human whole ductal carcinoma in situ.
Data processing Data was normalized using the GeneSpring version 12 software environment provided by Agilent. Probe spots were either marked as detected with errors lower than 3 times the signal, or if flagged were given a value of 0.1. Probe spots for the same miRNA were summed, individual arrays were log2 transformed, and subsequently 90th percentile normalized.
Submission date Jul 09, 2014
Last update date Jul 07, 2016
Contact name Robert Lesurf
Organization name Washington University in St. Louis
Department McDonnell Genome Institute
Lab Griffith Lab
Street address 4444 Forest Park Ave
City St. Louis
State/province MO
ZIP/Postal code 63112
Country USA
Platform ID GPL15019
Series (2)
GSE59247 Molecular features of subtype-specific progression from ductal carcinoma in situ to invasive breast cancer [miRNA data]
GSE59248 Molecular features of subtype-specific progression from ductal carcinoma in situ to invasive breast cancer

Data table header descriptions
VALUE log2 transformed 90th percentile normalized.

Data table
Blank -10.937395
NC1_00000197 -10.937395
NC1_00000215 -10.937395
NC2_00079215 -10.937395
NC2_00092197 -10.937395
NC2_00106057 -10.937395
NC2_00122731 -10.937395
NegativeControl -10.937395
bkv-miR-B1-3p -10.937395
bkv-miR-B1-5p -10.937395
dmr_285 2.1202703
dmr_3 6.2829976
dmr_308 -10.937395
dmr_316 -10.937395
dmr_31a 1.222281
dmr_6 5.3822703
ebv-miR-BART1-3p -10.937395
ebv-miR-BART1-5p -10.937395
ebv-miR-BART10 -10.937395
ebv-miR-BART10* -10.937395

Total number of rows: 1368

Table truncated, full table size 32 Kbytes.

Supplementary file Size Download File type/resource
GSM1431375_230312_253118112250_S01_miRNA_107_Sep09_patch_1_4.txt.gz 8.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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