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Sample GSM1429751 Query DataSets for GSM1429751
Status Public on Jun 26, 2017
Title EM-mRNA-1
Sample type SRA
 
Source name hydatid cyst
Organism Echinococcus multilocularis
Characteristics larvel stage: protoscoleces
Extracted molecule total RNA
Extraction protocol Total RNA from the parasites was purified with Trizol reagent and contaminating gemomic DNA was removed by the RNase-Free DNase Set. Polyadenylated RNA were isolated from total RNA.
The mRNA was interrupted into short fragments by adding fragmentation buffer as described (Illumina RNA-seq kit, part no. 1004898). Taking these short fragments as templates, random hexamer primers were used to synthesize the first-strand cDNA. The second-strand cDNA was synthesized using buffer, dNTPs, RNase H and DNA polymerase I, respectively. Short fragments were purified and double strand cDNAs were end-repaired according to manufacturer-recommended protocols (https://icom.illumina.com/), and then were connected with Illumina adapters (Illumina RNA-seq kit, part no. 1004898). The fragments were first amplified by PCR. Purified cDNA fragments were pooled and indexed and were loaded onto one lane of an Illumina HiSeqâ„¢ 2000 instrument. Pair-end 100 sequencing cycles were carried out.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description Sample 3
Data processing For mRNA sequencing,low quality and adapter sequences were removed from the Illumina reads by an in-house Perl script.
For mRNA sequencing,high-quality reads were mapped onto the E. granulosus and E. multilocularis genome v3 downloaded from http://www.sanger.ac.uk/ by tophat v2.0.4.
mRNA transcript sequences were assembled by Cufflinks v2.0.2. Then Cuffdiff was used to find significant changes of expression.
Functional annotation and classification were analysed by using KEGG and Gene Ontology databases.
Genome_build: E. granulosus and E. multilocularis genome v3 downloaded from http://www.sanger.ac.uk/
Supplementary_files_format_and_content: The .xlsx file reports FPKMs.
 
Submission date Jul 08, 2014
Last update date May 15, 2019
Contact name xiaosu zhou
E-mail(s) xiaosu_joe@163.com
Organization name Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Street address Dong Dan San Tiao 9
City Beijing
ZIP/Postal code 100730
Country China
 
Platform ID GPL18906
Series (1)
GSE59173 Transcriptome analysis of mRNA and microRNA in the cestodes Echinococcus granulosus and Echinococcus multilocularis
Relations
BioSample SAMN02904722
SRA SRX647644

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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