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Sample GSM1427137 Query DataSets for GSM1427137
Status Public on Jan 01, 2015
Title RNAseq_F-shEZH1_rep3
Sample type SRA
Source name Human primary fetal live proerythroblasts (ProEs), shEZH1
Organism Homo sapiens
Characteristics tissue: fetal liver
cell type: CD34+ HSPC-derived proerythroblasts
shRNA: shEZH1
Treatment protocol NA
Growth protocol Primary maturing fetal liver erythroblasts were generated ex vivo using a serum-free two-phase liquid culture system.
Extracted molecule total RNA
Extraction protocol For RNA-seq analysis using the Illumina HiSeq 2500, 0.1-2 ug of total RNA was processed for library generation using the TruSeq RNA Sample Preparation v2 Kit (Illumina) following the manufacturer's protocol.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Description mRNA
Data processing Sequencing reads were aligned to human genome assembly hg18 (NCBI version 36) by TopHat.
The number of reads aligned to RefSeq annotated genes' exons were counted and further normalized to be of unit RPKM (number of reads per kilobase pair per million total reads).
Differential gene expression analyses were performed using DEseq.
Genome_build: hg18 (NCBI36)
Supplementary_files_format_and_content: Tab-delimited text file of gene RPKM values.
Submission date Jul 03, 2014
Last update date May 15, 2019
Contact name Jian Xu
Phone 214-648-6125
Organization name UT Southwestern Medical Center
Department Children's Research Institute
Street address 6000 Harry Hines Blvd. NL12.138B
City Dallas
State/province TX
ZIP/Postal code 75235
Country USA
Platform ID GPL16791
Series (2)
GSE59089 RNA-seq transcriptional profiling in primary human erythroid progenitor cells upon shRNA-mediated knockdown of PRC2 core subunits
GSE59090 ChIP-seq and RNA-seq analysis of PRC2 core subunits in primary human erythroid progenitor cells
BioSample SAMN02902846
SRA SRX646143

Supplementary file Size Download File type/resource
GSM1427137_F-shEZH1_rep3_refseq_gene.rpkm.gz 322.1 Kb (ftp)(http) RPKM
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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