|Public on Jan 01, 2015
|Human primary fetal live proerythroblasts (ProEs), shEZH1
|tissue: fetal liver
cell type: CD34+ HSPC-derived proerythroblasts
|Primary maturing fetal liver erythroblasts were generated ex vivo using a serum-free two-phase liquid culture system.
|For RNA-seq analysis using the Illumina HiSeq 2500, 0.1-2 ug of total RNA was processed for library generation using the TruSeq RNA Sample Preparation v2 Kit (Illumina) following the manufacturer's protocol.
|Illumina HiSeq 2500
|Sequencing reads were aligned to human genome assembly hg18 (NCBI version 36) by TopHat.
The number of reads aligned to RefSeq annotated genes' exons were counted and further normalized to be of unit RPKM (number of reads per kilobase pair per million total reads).
Differential gene expression analyses were performed using DEseq.
Genome_build: hg18 (NCBI36)
Supplementary_files_format_and_content: Tab-delimited text file of gene RPKM values.
|Jul 03, 2014
|Last update date
|May 15, 2019
|UT Southwestern Medical Center
|Children's Research Institute
|6000 Harry Hines Blvd. NL12.138B
|RNA-seq transcriptional profiling in primary human erythroid progenitor cells upon shRNA-mediated knockdown of PRC2 core subunits
|ChIP-seq and RNA-seq analysis of PRC2 core subunits in primary human erythroid progenitor cells