|
| Status |
Public on Feb 09, 2017 |
| Title |
CTR2 |
| Sample type |
RNA |
| |
|
| Source name |
human gastric cancer
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MKN45
cell type: gastric cancer cells
cell line source: stomach, liver metastasis, epithelial-like adenocarcinoma in a 62 year-old Japanese female
treated with: none (control)
|
| Treatment protocol |
Cells were cultured in DMEM with 10% FBS. For fucoidan treatment, culture medium was replaced with fresh medium mixed with fucoidan (1 mg/ml), and cells were cultured until the assay.
|
| Growth protocol |
Cell growth assay was performed by counting cell numbers using a hemocytometer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was collected from cultured MKN45 cells using the RNeasy mini kit (Qiagen, Hilden, Germany).
|
| Label |
Cy3
|
| Label protocol |
Agilent one-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution.
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 039494_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Jun 24, 2014 |
| Last update date |
Apr 23, 2018 |
| Contact name |
Miwa Yoshimoto |
| E-mail(s) |
m-yoshimoto@med.tottori-u.ac.jp
|
| Organization name |
Tottori University
|
| Street address |
36-1 Nishi-cho
|
| City |
Yonago |
| ZIP/Postal code |
683-8504 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16699 |
| Series (1) |
| GSE58774 |
Anti-proliferation activity of fucoidan on MKN45 gastric cancer cells and downregulation of phosphorylated ASK1, a cell-cycle-regulated cyclin-dependent kinase |
|
| Relations |
| Reanalyzed by |
GSE113533 |
