|
| Status |
Public on Jan 20, 2015 |
| Title |
5211_CD4_Memory_48 [Affymetrix] |
| Sample type |
RNA |
| |
|
| Source name |
Human Memory CD4 T Cell
|
| Organism |
Homo sapiens |
| Characteristics |
activation: anti-CD3/CD28 beads, 48 hours
|
| Treatment protocol |
Purified naïve and memory CD4 T cells were cultured in RPMI supplemented with 10% FBS, 100 U/ml penicillin and 100mg/ml streptomycin at 37C and 5% CO2 with anti-CD3/CD28 activator beads (Invitrogen) for 0 or 48 hours.
|
| Growth protocol |
Naïve and memory CD4 cells were negatively selected using magnetic beads (Stem Cell Technologies).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from cell pellets using an All Prep kit (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared using the Ambion WT Expression Kit (Ambion Life Technologies) following manufacturer's instructions. cDNA was fragmented and labeled with GeneChip WT Terminial Labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following labelling, 2.5 ug of cDNA were hybridized for 17 h at 48C on HuGene 1.1ST peg arraya (Affymetrix)
|
| Scan protocol |
Washing and staining and scanning done per the Gene Titan MC protocol for HuGene 1.1ST peg arrays.
|
| Description |
Gene expression data for anti-CD3/CD28 activated human memory CD4 T cells
|
| Data processing |
The data were analyzed with Partek Genomics Suite (Partek Incorporated) and was Robust Multi-array Average (RMA) normalized
|
| |
|
| Submission date |
Jun 19, 2014 |
| Last update date |
Jan 23, 2015 |
| Contact name |
Daniel Salomon |
| E-mail(s) |
dsalomon@scripps.edu
|
| Organization name |
The Scripps Research Institute
|
| Department |
Molecular & Experimental Medicine
|
| Lab |
Salomon
|
| Street address |
10550 N Torrey Pines Rd, MEM-241
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL11532 |
| Series (2) |
| GSE58684 |
Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation [Affymetrix] |
| GSE59860 |
Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation |
|
