|
| Status |
Public on Apr 02, 2015 |
| Title |
Ins |
| Sample type |
SRA |
| |
|
| Source name |
GC
|
| Organism |
Homo sapiens |
| Characteristics |
transduction: Ins AR-HA
cell type: primary ovarian granulosa cells
chip-antibody: HA
|
| Treatment protocol |
DHT treatment
|
| Growth protocol |
GCs of infertile women with tubal factor were isolated and cultured.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and AR-HA-DNA complexes were isolated with antibody.
Libraries were prepared according to Illumina's instructions.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Basecalls performed using HCS1.5.15.1+RTA1.13.48+OLB1.9.4.
Sequenced reads were mapped to the human genome (hg19) by SOAP2.21.
Raw data were filtered by SOAPnuke, one read will be removed if it matches any condition shows bellow: 1. contain adapter read；2. N base is more than 10% of read；3. bases with quality low than 20 is more than 50% of read.
Peaks were called using MACS-1.4.0alpha2.
Genome_build: hg19
Supplementary_files_format_and_content: Bed files were generated by MACS after soap alignment, the columns represent chromsome, start positon and end position in chromsome respectively.
|
| |
|
| Submission date |
Jun 18, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Fangfang Wang |
| E-mail(s) |
doctorwangfangfang2011@gmail.com
|
| Organization name |
Zhejiang University
|
| Street address |
No.1 Xueshi Road.
|
| City |
Hangzhou |
| ZIP/Postal code |
310006 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE58628 |
Genome-wide maps of AR recruitment in primary human ovarian granulosa cells (GCs) transduced with androgen receptor (AR) alternative splice variant (ASV) |
|
| Relations |
| BioSample |
SAMN02866219 |
| SRA |
SRX610673 |
