|
| Status |
Public on Aug 17, 2014 |
| Title |
Patient 1, week 12 |
| Sample type |
RNA |
| |
|
| Source name |
Patient 1, week 12
|
| Organism |
Homo sapiens |
| Characteristics |
disease: alopecia areata
tissue: skin
treatment: ruxolinitib
weeks of treatment: 12
|
| Treatment protocol |
scalp biopsy specimens were fixed using PAXgene kits
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PreAnalytiX - PAXgene Tissue miRNA Kit
|
| Label |
biotin
|
| Label protocol |
NuGen Encore Biotin Protocol using 3.75 ug cDNA from NuGen Ovation RNA Amplification V2 system
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.75 ug of cDNA was hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G3000.
|
| Data processing |
The data were analyzed with Bioconductor/R and were RMA normalized.
|
| |
|
| Submission date |
Jun 17, 2014 |
| Last update date |
Aug 17, 2014 |
| Contact name |
Ali Jabbari |
| Organization name |
University of Iowa
|
| Street address |
200 Hawkins Dr, 40025 PFP
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE58573 |
First ruxolitinib treatment of human alopecia areata patients |
|
