|
| Status |
Public on Nov 19, 2015 |
| Title |
SARS_VEROlowMOI_24hr_1 |
| Sample type |
SRA |
| |
|
| Source name |
Vero E6 cells
|
| Organism |
Chlorocebus sabaeus |
| Characteristics |
cell line: Vero E6
|
| Treatment protocol |
RNA was isolated at 24 and 48 hours post infection at an MOI of 0.1 and 3 with each virus.
|
| Growth protocol |
Vero E6 cells were grown in MEM with 10% Fetal Bovine Serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol from cells infected in culture.
Poly-A RNA was isolated and indexed libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
NOT PROVIDED
Genome_build: NOT PROVIDED
Supplementary_files_format_and_content: NOT PROVIDED
|
| |
|
| Submission date |
Jun 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
David A Rasko |
| E-mail(s) |
drasko@som.umaryland.edu
|
| Phone |
410 706 6774
|
| Organization name |
University of Maryland School of Medicine
|
| Department |
Istitute for Genome Sciences/Microbiology and Immunology
|
| Street address |
801 W. Baltimore Street, Room 619
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL18770 |
| Series (2) |
| GSE56189 |
Transcriptomic Analysis Of The Novel Middle East Respiratory Syndrome Coronavirus (MERS-CoV) |
| GSE56193 |
Transcriptomic analysis of the Novel Middle East Respiratory Syndrome Coronavirus (African green monkey, Vero E6 cells) |
|
| Relations |
| SRA |
SRX492374 |
| SRA |
SRX492375 |
| BioSample |
SAMN02689470 |
