Tissue: bone marrow-derived mesenchymal stem cell (Cambrex Lot#4F0312)
Biomaterial provider
Dr Biagio Saitta, Coriell Institute for Medical Research, 403 Haddon Ave, Camden, NJ 08103, USA, bsaitta@coriell.org http://www.coriell.org/ Coriell
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from cells in culture using the RNAeasy Mini Kit (Qiagen), according to manufacturer's protocol
Label
Biotin
Label protocol
Microarray target was prepared following the Affymetrix protocol. In brief, cDNA was synthesized from total RNA using the SuperScript II double-stranded cDNA synthesis kit (Invitrogen), followed by in vitro transcription, where labeled cRNA was generated (Enzo).
Hybridization protocol
Hybridization was carried out according to Affymetrix protocol.
Description
none
Data processing
RMA method normalization implemented in BioConductor
