|
| Status |
Public on May 27, 2014 |
| Title |
VN30259.Pre_sample 1 |
| Sample type |
RNA |
| |
|
| Source name |
Bronchial Brush
|
| Organism |
Macaca mulatta |
| Characteristics |
animal number: 4
influenza strain: VN30259
time (days post infection): Pre
tissue: Bronchial Brush
|
| Treatment protocol |
Tissue from brushes were placed in the RNA stabilization reagent RNAlater (Ambion, CA) and stored at -80°C. All tissues were thawed together and homogenized (2 minutes at 30Hz) by using a TissueLyser (Qiagen, Hilden, Germany), following the manufacturer’s instructions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from bronchial brushes with the RNeasy Mini Kit (Qiagen, Hilden, Germany) following the manufacturer's recommendation.
|
| Label |
Cy3
|
| Label protocol |
Using the Quick Amp Labeling Kit, T7 RNA polymerase was used to simultaneously amplify target material and incorporate cyanine 3-labeled CTP to prepare Cy3-labeled cRNA.
|
| |
|
| Hybridization protocol |
Using Agilents Gene Expression Hybridizatoin kit #4 and Gene Expression wash pack #5, Cy3-labeled cRNA preparations were hybridized with rhesus macaque arrays (Agilent Microarray Design Identification Number 015421) for 17 h at 65°C.
|
| Scan protocol |
Immediately after washing, Feature Extraction Software, version 7 (Agilent Technologies) was used for image analysis and data extraction.
|
| Description |
SAMPLE 1
|
| Data processing |
Per chip and per gene normalization was performed using the LIMMA package in R.
|
| |
|
| Submission date |
May 27, 2014 |
| Last update date |
May 28, 2014 |
| Contact name |
Yoshihiro Kawaoka |
| Organization name |
Japanese Science and Technology Agency
|
| Department |
ERATO Influenza-induced Host Responses Project
|
| Lab |
Kawaoka
|
| Street address |
Shirokanedai 4-6-1
|
| City |
Minato |
| State/province |
Tokyo |
| ZIP/Postal code |
1088639 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14569 |
| Series (1) |
| GSE57970 |
Disease severity is associated with differential gene expression at the early and late phases of infection in non-human primates infected with different H5N1 highly pathogenic avian influenza viruses |
|
